Proteintech, 83089-1-RR, COPA Recombinant monoclonal antibody

Size: 20ul / 100ul The COPA (83089-1-RR) by Proteintech is a Recombinant antibody targeting COPA in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 83089-1-RR targets COPA in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells, NIH/3T3 cells, MCF-7 cells, C2C12 cells Positive IHC detected in: mouse liver tissue, human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information COPA, also named HEP-COP and Alpha-COP, is coatomer subunit alpha. The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag34207 Product name: Recombinant human COPA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 925-1224 aa of NM_004371 Sequence: QLPVDHILAGSFETAMRLLHDQVGVIQFGPYKQLFLQTYARGRTTYQALPCLPSMYGYPNRNWKDAGLKNGVPAVGLKLNDLIQRLQLCYQLTTVGKFEEAVEKFRSILLSVPLLVVDNKQEIAEAQQLITICREYIVGLSVETERKKLPKETLEQQKRICEMAAYFTHSNLQPVHMILVLRTALNLFFKLKNFKTAATFARRLLELGPKPEVAQQTRKILSACEKNPTDAYQLNYDMHNPFDICAASYRPIYRGKPVEKCPLSGACYSPEFKGQICRVTTVTEIGKDVIGLRISPLQFR Predict reactive species Full Name: coatomer protein complex, subunit alpha Calculated Molecular Weight: 138 kDa Observed Molecular Weight: 150 kDa GenBank Accession Number: NM_004371 Gene Symbol: COPA Gene ID (NCBI): 1314 RRID: AB_3670804 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P53621 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.