Proteintech, 83095-1-RR, Acetyl-Histone H4 (Lys12) Recombinant monoclonal antibody

Size: 20ul / 100ul The Acetyl-Histone H4 (Lys12) (83095-1-RR) by Proteintech is a Recombinant antibody targeting Acetyl-Histone H4 (Lys12) in WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications with reactivity to human, mouse, rat samples 83095-1-RR targets Acetyl-Histone H4 (Lys12) in WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Trichostatin A treated NIH/3T3 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells Positive IHC detected in: mouse small intestine tissue, human placenta tissue, mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Caco-2 cells, Trichostatin A treated NIH/3T3 cells Positive Dot Blot detected in: peptide Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600 DOT BLOT: DOT BLOT : 1:10-1:100 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 1, H4a Observed Molecular Weight: 12 kDa GenBank Accession Number: BC069654 Gene Symbol: HIST1H4A Gene ID (NCBI): 8359 RRID: AB_3670809 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P62805 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.