Proteintech, 83104-1-RR, ANT1/2 Recombinant monoclonal antibody

Size: 20ul / 100ul The ANT1/2 (83104-1-RR) by Proteintech is a Recombinant antibody targeting ANT1/2 in WB, ELISA applications with reactivity to human, mouse, rat samples 83104-1-RR targets ANT1/2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, C6 cells, NIH/3T3 cells, COS-7 cells, Neuro-2a cells, rat liver tissue, mouse liver tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag11956 Product name: Recombinant human SLC25A5 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-298 aa of BC056160 Sequence: MTDAAVSFAKDFLAGGVAAAISKTAVAPIERVKLLLQVQHASKQITADKQYKGIIDCVVRIPKEQGVLSFWRGNLANVIRYFPTQALNFAFKDKYKQIFLGGVDKRTQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKAGAEREFRGLGDCLVKIYKSDGIKGLYQGFNVSVQGIIIYRAAYFGIYDTAKGMLPDPKNTHIVISWMIAQTVTAVAGLTSYPFDTVRRRMMMQSGRKGTDIMYTGTLDCWRKIARDEGGKAFFKGAWSNVLRGMGGAFVLVLYDEIKKYT Predict reactive species Full Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5 Calculated Molecular Weight: 298 aa, 33 kDa Observed Molecular Weight: 32 kDa GenBank Accession Number: BC056160 Gene Symbol: SLC25A5 Gene ID (NCBI): 292 RRID: AB_3670817 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P05141 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.