Proteintech, 83131-8-RR, NLRP10 Recombinant monoclonal antibody

Size: 20ul / 100ul The NLRP10 (83131-8-RR) by Proteintech is a Recombinant antibody targeting NLRP10 in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 83131-8-RR targets NLRP10 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse heart tissue Positive FC (Intra) detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information NLRP10 is a member of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). NLRP10 likely plays a regulatory role in the innate immune system. The protein belongs to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag26402 Product name: Recombinant human NLRP10 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-180 aa of BC104957 Sequence: MAMAKARKPREALLWALSDLEENDFKKLKFYLRDMTLSEGQPPLARGELEGLIPVDLAELLISKYGEKEAVKVVLKGLKVMNLLELVDQLSHICLHDYREVYREHVRCLEEWQEAGVNGRYNQVLLVAKPSSESPESLACPFPEQELESVTVEALFDSGEKPSLAPSLVVLQGSAGTGKT Predict reactive species Full Name: NLR family, pyrin domain containing 10 Calculated Molecular Weight: 655 aa, 75 kDa Observed Molecular Weight: 75 kDa GenBank Accession Number: BC104957 Gene Symbol: NLRP10 Gene ID (NCBI): 338322 RRID: AB_3670834 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q86W26 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.