Proteintech, 83154-1-RR, INTS5 Recombinant monoclonal antibody

Size: 20ul / 100ul The INTS5 (83154-1-RR) by Proteintech is a Recombinant antibody targeting INTS5 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 83154-1-RR targets INTS5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, NIH/3T3 cells, HCT-116 cells, HT-29 cells, SKOV-3 cells Positive IF/ICC detected in: U2OS cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information INTS5, also named as INT5 and KIAA1698, is a component of the Integrator complex which involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag5129 Product name: Recombinant human INTS5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-322 aa of BC060841 Sequence: MSALCDPPGAPGPPGPAPATHGPAPLSAQELSQEIKAFLTGVDPILGHQLSAREHARCGLLLLRSLPPARAAVLDHLRGVFDESVRAHLAALDETPVAGPPHLRPPPPSHVPAGGPGLEDVVQEVQQVLSEFIRANPKAWAPVISAWSIDLMGQLSSTYSGQHQRVPHATGALNELLQLWMGCRATRTLMDIYVQCLSALIGSCPDACVDALLDTSVQHSPHFDWVVAHIGSSFPGTIISRVLSCGLKDFCVHGGAGGGAGSSGGSSSQTPSTDPFPGSPAIPAEKRVPKIASVVGILGHLASRHGDSIRRELLRMFHDSLA Predict reactive species Full Name: integrator complex subunit 5 Calculated Molecular Weight: 108 kDa Observed Molecular Weight: 108 kDa GenBank Accession Number: BC060841 Gene Symbol: INTS5 Gene ID (NCBI): 80789 RRID: AB_3670848 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q6P9B9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.