Proteintech, 83212-4-RR, NCBP1/CBP80 Recombinant monoclonal antibody

Size: 20ul / 100ul The NCBP1/CBP80 (83212-4-RR) by Proteintech is a Recombinant antibody targeting NCBP1/CBP80 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, rat samples 83212-4-RR targets NCBP1/CBP80 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: Jurkat cells, HEK-293 cells, HeLa cells, HepG2 cells, A431 cells, NCI-H1299 cells, PC-12 cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing. Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag0496 Product name: Recombinant human CBP80 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 46-350 aa of BC001450 Sequence: LESNLEGLAGVLEADLPNYKSKILRLLCTVARLLPEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHVIAAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANTESYLKRRQKTHVPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDSVYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSHWKERKTCAAQLVSYPGKNKIPLNYH Predict reactive species Full Name: nuclear cap binding protein subunit 1, 80kDa Calculated Molecular Weight: 92 kDa Observed Molecular Weight: 80 kDa GenBank Accession Number: BC001450 Gene Symbol: NCBP1 Gene ID (NCBI): 4686 RRID: AB_3670896 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q09161 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.