Proteintech, 83227-1-RR, TCP11L2 Recombinant monoclonal antibody

Size: 20ul / 100ul The TCP11L2 (83227-1-RR) by Proteintech is a Recombinant antibody targeting TCP11L2 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, canine samples 83227-1-RR targets TCP11L2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, canine samples. Tested Applications Positive WB detected in: Jurkat cells, MDCK cells, U251 cells Positive IF/ICC detected in: U-251 cells Positive FC (Intra) detected in: A431 cells, SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information TCP11L2 is a protein containing a serine-rich region in its N-terminal region. It is a member of the TCP11 family, which includes SOK1, TCP11L1, and TCP11L2. TCP11L2 has a role in the migration and differentiation of skeletal muscle-derived satellite cells. It has been shown that Tcp11l2 expression gradually increased during the differentiation of bovine muscle-derived satellite cells. (PMID: 32017087) Specification Tested Reactivity: human, canine Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag11185 Product name: Recombinant human TCP11L2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 169-519 aa of BC033617 Sequence: EHSAVDIQGLANYVISTMGKLCAPVRDNDIRELKATGNIVEVLRQIFHVLDLMQMDMANFTIMSLRPHLQRQLVEYERTKFQEILEETPSALDQTTEWIKESVNEELFSLSESALTPGAENTSKPSLSPTLVLNNSYLKLLQWDYQKKELPETLMTDGARLQELTEKLNQLKIIACLSLITNNMVGAITGGLPELASRLTRISAVLLEGMNKETFNLKEVLNSIGIQTCVEVNKTLMERGLPTLNAEIQANLIGQFSSIEEEDNPIWSLIDKRIKLYMRRLLCLPSPQKCMPPMPGGLAVIQQELEALGSQYANIVNLNKQVYGPFYANILRKLLFNEEAMGKVDASPPTN Predict reactive species Full Name: t-complex 11 (mouse)-like 2 Calculated Molecular Weight: 519 aa, 58 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC033617 Gene Symbol: TCP11L2 Gene ID (NCBI): 255394 RRID: AB_3670910 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q8N4U5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.