Proteintech, 83228-1-RR, SULT1B1 Recombinant monoclonal antibody

Size: 20ul / 100ul The SULT1B1 (83228-1-RR) by Proteintech is a Recombinant antibody targeting SULT1B1 in WB, IHC, FC (Intra), ELISA applications with reactivity to human, mouse samples 83228-1-RR targets SULT1B1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse colon tissue Positive IHC detected in: mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Sulfotransferase 1B1 (SULT1B1) is expressed at highest levels throughout the human colon and small intestine but can also be found at moderate levels in human liver, kidney, and white blood cells (PMID: 28084139). Certain SULT1B1 SNPs may influence the activity of thyroid hormones and the mutagenicity of polycyclic aromatic hydrocarbons (PMID: 34107842). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag9009 Product name: Recombinant human SULT1B1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-296 aa of BC010895 Sequence: MLSPKDILRKDLKLVHGYPMTCAFASNWEKIEQFHSRPDDIVIATYPKSGTTWVSEIIDMILNDGDIEKCKRGFITEKVPMLEMTLPGLRTSGIEQLEKNPSPRIVKTHLPTDLLPKSFWENNCKMIYLARNAKDVSVSYYHFDLMNNLQPFPGTWEEYLEKFLTGKVAYGSWFTHVKNWWKRKEEHPILFLYYEDMKENPKEEIKKIIRFLEKNLNDEILDRIIHHTSFEVMKDNPLVNYTHLPTTVMDHSKSPFMRKGTAGDWKNYFTVAQNEKFDAIYETEMSKTALQFRTEI Predict reactive species Full Name: sulfotransferase family, cytosolic, 1B, member 1 Calculated Molecular Weight: 296 aa, 35 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: BC010895 Gene Symbol: SULT1B1 Gene ID (NCBI): 27284 RRID: AB_3670911 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O43704 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.