Proteintech, 83332-3-RR, Phospho-HSP27 (Ser15) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-HSP27 (Ser15) (83332-3-RR) by Proteintech is a Recombinant antibody targeting Phospho-HSP27 (Ser15) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 83332-3-RR targets Phospho-HSP27 (Ser15) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Anisomycin treated HeLa cells Positive IF/ICC detected in: Anisomycin treated HeLa cells Positive FC (Intra) detected in: Anisomycin treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information HSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: heat shock 27kDa protein 1 Calculated Molecular Weight: 19-23 kDa Observed Molecular Weight: 27 kDa GenBank Accession Number: BC012768 Gene Symbol: HSPB1 Gene ID (NCBI): 3315 RRID: AB_3670996 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P04792 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.