Proteintech, 83342-1-RR, RHOBTB1 Recombinant monoclonal antibody

Size: 20ul / 100ul The RHOBTB1 (83342-1-RR) by Proteintech is a Recombinant antibody targeting RHOBTB1 in WB, ELISA applications with reactivity to Human samples 83342-1-RR targets RHOBTB1 in WB, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, U-251 cells, HT-29 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Rho-related BTB domain containing protein 1 (RhoBTB1) is also named as KIAA0740. RhoBTB1 is an atypical Rho GTPase with two BTB domains in addition to its Rho domain. RhoBTB1 is a substrate for ROCK1, and mutation of putative phosphorylation sites reduces its association with Cullin3, a scaffold for ubiquitin ligases (PMID: 31431478). RHOBTB1 is a novel PPARγ gene target in vascular smooth muscle cells that mediates the protective effect of PPARγ by serving as a substrate adaptor between the Cullin-3 RING ubiquitin ligase and phosphodiesterase 5, thus restraining its activity through ubiquitination and proteasomal degradation (PMID: 31789920). Specification Tested Reactivity: Human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag3365 Product name: Recombinant human RHOBTB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-344 aa of BC032848 Sequence: MDADMDYERPNVETIKCVVVGDNAVGKTRLICARACNTTLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDEVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLNHVKSMWYPEIKHFCPRTPVILVGCQLDLRYADLEAVNRARRPLARPIKRGDILPPEKGREVAKELGLPYYETSVFDQFGIKDVFDNAIRAALISRRHLQFWKSHLKKVQKPLLQAPFLPPKAPPPVIKIPECPSMGTNEAACLLDNPLCADVLFILQDQEHIFAHRIYLATSSSKFYDLFLMECEESPNGSEGACEKEKQSRDFQGRILSVDPEEEREEGPPRIPQAD Predict reactive species Full Name: Rho-related BTB domain containing 1 Calculated Molecular Weight: 696 aa, 79 kDa Observed Molecular Weight: 79 kDa GenBank Accession Number: BC032848 Gene Symbol: RHOBTB1 Gene ID (NCBI): 9886 RRID: AB_3671003 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O94844 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.