Proteintech, 83363-1-RR, RBM20 Recombinant monoclonal antibody

Size: 20ul / 100ul The RBM20 (83363-1-RR) by Proteintech is a Recombinant antibody targeting RBM20 in WB, FC (Intra), ELISA applications with reactivity to human samples 83363-1-RR targets RBM20 in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293T cells, K-562 cells, TT cells, A549 cells Positive FC (Intra) detected in: K-562 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information RBM20 (RNA binding motif protein 20) is a heart- and skeletal muscle- nriched splicing factor that controls tissue-specific isoform expression of many other genes besides TTN, including sarcomeric genes such myomesin 1, but also Ca2+- and ion-handling genes such as CAMK2D (calcium/calmodulin-dependent kinase II-delta) and RYR2 (ryanodine receptor 2) (PMID: 29650543). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag34322 Product name: Recombinant human RBM20 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 667-840 aa of NM_001134363 Sequence: ADWGNGRDSWEHSPYARREEERDPAPWRDNGDDKRDRMDPWAHDRKHHPRQLDKAELDERPEGGRPHREKYPRSGSPNLPHSVSSYKSREDGYYRKEPKAKSDKYLKQQQDAPGRSRRKDEARLRESRHPHPDDSGKEDGLGPKVTRAPEGAKAKQNEKNKTKRTDRDQEGADD Predict reactive species Full Name: RNA binding motif protein 20 Calculated Molecular Weight: 134 kDa Observed Molecular Weight: 190 kDa GenBank Accession Number: NM_001134363 Gene Symbol: RBM20 Gene ID (NCBI): 282996 RRID: AB_3671017 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q5T481 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.