Proteintech, 83401-4-RR, TSSK4 Recombinant monoclonal antibody

Size: 20ul / 100ul The TSSK4 (83401-4-RR) by Proteintech is a Recombinant antibody targeting TSSK4 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 83401-4-RR targets TSSK4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, HT-29 cells, COLO 320 cells, mouse testis tissue, mouse colon tissue, rat testis tissue Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse testis tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information TSSK4 (Testis-specific serine/threonine-protein kinase 4), testis-specific serine/threonine protein kinase 4, is a member of the testis-specific serine/threonine protein kinase family. It has autophosphorylation activity and self-binding properties; it can interact with and phosphorylate other proteins, e.g., it can bind to and phosphorylate Odf2. It's reported that TSSK4 plays an important role in spermatogenesis, TSSK4 knockout male mice develop reduced sperm motility and reduced fertility. In addition, TSSK4 regulates lung fibrosis by promoting the loss of alveolar epithelial type II cells through the HSP90-AKT signaling pathway. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag16205 Product name: Recombinant human TSSK4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 81-252 aa of BC111088 Sequence: DKWENVKISDFGFAKMVPSNQPVGCSPSYRQVNCFSHLSQTYCGSFAYACPEILRGLPYNPFLSDTWSMGVILYTLVVAHLPFDDTNLKKLLRETQKEVTFPANHTISQECKNLILQMLRQATKRATILDIIKDSWVLKFQPEQPTHEIRLLEAMCQLHNTTKQHQSLQITT Predict reactive species Full Name: testis-specific serine kinase 4 Calculated Molecular Weight: 338 aa, 37 kDa Observed Molecular Weight: 38-45 kDa GenBank Accession Number: BC111088 Gene Symbol: TSSK4 Gene ID (NCBI): 283629 RRID: AB_3671052 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q6SA08 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.