Proteintech, 83422-1-RR, Cystatin C Recombinant monoclonal antibody

Size: 20ul / 100ul The Cystatin C (83422-1-RR) by Proteintech is a Recombinant antibody targeting Cystatin C in WB, IHC, ELISA applications with reactivity to human, mouse samples 83422-1-RR targets Cystatin C in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: RAW 264.7 cells, mouse brain tissue Positive IHC detected in: mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidney. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Fusion Protein Predict reactive species Full Name: cystatin C Calculated Molecular Weight: 15KD Observed Molecular Weight: 13-16 kDa GenBank Accession Number: NM_009976.4 Gene Symbol: Cystatin C Gene ID (NCBI): 13010 RRID: AB_3671071 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P21460 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.