Proteintech, 83428-1-RR, Ferritin heavy chain Recombinant monoclonal antibody

Size: 20ul / 100ul The Ferritin heavy chain (83428-1-RR) by Proteintech is a Recombinant antibody targeting Ferritin heavy chain in WB, IF/ICC, ELISA applications with reactivity to human samples 83428-1-RR targets Ferritin heavy chain in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, human liver tissue, human placenta tissue Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag2290 Product name: Recombinant human FTH1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-183 aa of BC000857 Sequence: MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES Predict reactive species Full Name: ferritin, heavy polypeptide 1 Calculated Molecular Weight: 183 aa, 21 kDa Observed Molecular Weight: 21 kDa GenBank Accession Number: BC000857 Gene Symbol: FTH1 Gene ID (NCBI): 2495 RRID: AB_3671075 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P02794 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.