Proteintech, 83442-6-RR, AGA Recombinant monoclonal antibody

Size: 20ul / 100ul The AGA (83442-6-RR) by Proteintech is a Recombinant antibody targeting AGA in WB, IHC, ELISA applications with reactivity to human, mouse samples 83442-6-RR targets AGA in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: MCF-7 cells, HepG2 cells, mouse testis tissue Positive IHC detected in: mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information AGA(Aspartylglucosaminidase) is also named as N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase and glycosylasparaginase.The open reading frame of the readthrough transcript would indicate AGA close to 40 kDa. It can be cleaved into 2 chains:the 24 kDa α- and 17 kDa β-subunits of the wild-type AGA,in addition,an aberrant polypeptide with a mol.wt of 21 kDa is also detected as a product of proteolytic cleavage of the mutant precursor molecule(PMID: 9425233).AGA can exsit as a dimer with the mol.wt between 100 kDa and 49 kDa and His124 at an interface between two heterodimers of AGA is crucial for the thermodynamically stable oligomeric structure of AGA.(PMID:9737998). It can also exsit as a heterotetrameric protein of 88 kDa(PMID:1733831). Specification Tested Reactivity: human, mouse Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag10681 Product name: Recombinant human AGA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-346 aa of BC012392 Sequence: MARKSNLPVLLVPFLLCQALVRCSSPLPLVVNTWPFKNATEAAWRALASGGSALDAVESGCAMCEREQCDGSVGFGGSPDELGETTLDAMIMDGTTMDVGAVGDLRRIKNAIGVARKVLEHTTHTLLVGESATTFAQSMGFINEDLSTSASQALHSDWLARNCQPNYWRNVIPDPSKYCGPYKPPGILKQDIPIHKETEDDRGHDTIGMVVIHKTGHIAAGTSTNGIKFKIHGRVGDSPIPGAGAYADDTAGAAAATGNGDILMRFLPSYQAVEYMRRGEDPTIACQKVISRIQKHFPEFFGAVICANVTGSYGAACNKLSTFTQFSFMVYNSEKNQPTEEKVDCI Predict reactive species Full Name: aspartylglucosaminidase Calculated Molecular Weight: 346 aa, 37 kDa Observed Molecular Weight: 24 kDa GenBank Accession Number: BC012392 Gene Symbol: AGA Gene ID (NCBI): 175 RRID: AB_3671083 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P20933 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.