Proteintech, 83489-1-RR, ARID4A Recombinant monoclonal antibody

Size: 20ul / 100ul The ARID4A (83489-1-RR) by Proteintech is a Recombinant antibody targeting ARID4A in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 83489-1-RR targets ARID4A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells, DU 145 cells, LNCaP cells, SW 480 cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Positive FC (Intra) detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information ARID4A (AT-rich interaction domain 4A, also known as retinoblastoma-binding protein 1, RBBP1/RBP1) is a retinoblastoma (RB)-binding protein. ARID1A is an important subunit of the switching defective/sucrose nonfermenting (SWI/SNF) chromatin remodeling complex. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag33114 Product name: Recombinant human ARID4A protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 900-1105 aa of NM_002892.3 Sequence: FEQHFERENEGMPSLIAESNQCIQQLTSERFDSPAEETVNIPLKEDEDAMPLIGPETLVCHEVDLDDLDEKDKTSIEDVAVESSESNSLVSIPPALPPVVQHNFSVASPLTLSQDESRSVKSESDITIEVDSIAEESQEGLCERESANGFETNVASGTCSIIVQERESREKGQKRPSDGNSGLMAKKQKRTPKRTSAAAKNEKNGT Predict reactive species Full Name: AT rich interactive domain 4A (RBP1-like) Calculated Molecular Weight: 143 kDa Observed Molecular Weight: 205 kDa GenBank Accession Number: NM_002892.3 Gene Symbol: ARID4A Gene ID (NCBI): 5926 RRID: AB_3671115 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P29374 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.