Proteintech, 83510-1-RR, MED21 Recombinant monoclonal antibody

Size: 20ul / 100ul The MED21 (83510-1-RR) by Proteintech is a Recombinant antibody targeting MED21 in WB, FC (Intra), ELISA applications with reactivity to human samples 83510-1-RR targets MED21 in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, L02 cells, HEK-293T cells Positive FC (Intra) detected in: HeLa cells, U-87 MG cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information MED21, also known as SRB7 and SURB7,belongs to the Mediator complex subunit 21 family. MED21 is a small essential subunit of the middle domain of the Mediator, which is conserved in all eukaryotes. MED21 is thought to play an important role in transcriptional activation and repression. MED21 regulates the transcription of nearly all RNA polymerase II-dependent genes (PMID: 16758199, PMID: 30559736). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag8568 Product name: Recombinant human MED21 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-144 aa of BC008380 Sequence: MADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFNNIQTAINKDQPANPTEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQLKTRSGTHSQSLPDS Predict reactive species Full Name: mediator complex subunit 21 Calculated Molecular Weight: 144 aa, 16 kDa Observed Molecular Weight: 13-16 kDa GenBank Accession Number: BC008380 Gene Symbol: MED21 Gene ID (NCBI): 9412 RRID: AB_3671135 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13503 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.