Proteintech, 83533-1-RR, ERK1/2 Recombinant monoclonal antibody

Size: 20ul / 100ul The ERK1/2 (83533-1-RR) by Proteintech is a Recombinant antibody targeting ERK1/2 in WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 83533-1-RR targets ERK1/2 in WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MCF-7 cells, Jurkat cells, HEK-293 cells, HepG2 cells, C6 cells, NIH/3T3 cells, rat brain tissue Positive IHC-Autostainer detected in: human pancreas tissue Positive IHC detected in: human cervical cancer tissue, human intrahepatic cholangiocarcinoma tissue, human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:20000 Immunohistochemistry (IHC)-AUTOSTAINER: IHC-AUTOSTAINER : 1:50-1:500 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag20350 Product name: Recombinant human ERK1/2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-379 aa of BC013992 Sequence: MAAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYIVQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETARFQPGVLEAP Predict reactive species Full Name: mitogen-activated protein kinase 3 Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 38-44 kDa GenBank Accession Number: BC013992 Gene Symbol: ERK1 Gene ID (NCBI): 5595 RRID: AB_3671158 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P27361 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.