Proteintech, 83558-5-RR, IL-2 Recombinant monoclonal antibody

Size: 20ul / 100ul The IL-2 (83558-5-RR) by Proteintech is a Recombinant antibody targeting IL-2 in WB, ELISA applications with reactivity to human samples 83558-5-RR targets IL-2 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells Positive IF/ICC detected in: PMA, A23187 and Brefeldin A treated Jurkat cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg0828 Product name: Recombinant Human IL-2 protein (His Tag) Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 21-153 aa of BC070338 Sequence: APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT Predict reactive species Full Name: interleukin 2 Calculated Molecular Weight: 153 aa, 18 kDa Observed Molecular Weight: 15 kDa GenBank Accession Number: BC070338 Gene Symbol: IL2 Gene ID (NCBI): 3558 RRID: AB_3671174 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P60568 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.