Proteintech, 83583-1-RR, PDK4 Recombinant monoclonal antibody

Size: 20ul / 100ul The PDK4 (83583-1-RR) by Proteintech is a Recombinant antibody targeting PDK4 in WB, IHC, FC (Intra), ELISA applications with reactivity to human, mouse samples 83583-1-RR targets PDK4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse kidney tissue, mouse lung tissue Positive IHC detected in: mouse skeletal muscle tissue, mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the increase in free fatty acids that occurs during starvation. Free fatty acids can activate peroxisome proliferator-activated receptor α (PPARα), and activation of PPARα can promote PDK4 expression (PMID: 11554740). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag3629 Product name: Recombinant human PDK4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 61-412 aa of BC040239 Sequence: LPVRLANILKEIDILPTQLVNTSSVQLVKSWYIQSLMDLVEFHEKSPDDQKALSDFVDTLIKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFPDQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIVVLGKEDLTIKISDRGGGVPLRIIDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSLSGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQMSSEADDWCIPSREPKNLAKEVAM Predict reactive species Full Name: pyruvate dehydrogenase kinase, isozyme 4 Calculated Molecular Weight: 411 aa, 46 kDa Observed Molecular Weight: 46 kDa GenBank Accession Number: BC040239 Gene Symbol: PDK4 Gene ID (NCBI): 5166 RRID: AB_3671197 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q16654 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.