Proteintech, 83585-1-RR, LUC7L Recombinant monoclonal antibody

Size: 20ul / 100ul The LUC7L (83585-1-RR) by Proteintech is a Recombinant antibody targeting LUC7L in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 83585-1-RR targets LUC7L in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, U-251 cells, mouse brain tissue, rat brain tissue Positive IHC detected in: rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information LUC7L, also known as LUC7L1, belongs to the Luc7 family. LUC7L encodes a putative RNA binding protein similar to the yeast Luc7p subunit of the U1 snRNP splicing complex, which is normally required for 5` splice site selection (PMID: 15474286). LUC7L is widely expressed. LUC7L has 3 isoforms with the molecular mass of 38, 42 and 44 kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag9170 Product name: Recombinant human LUC7L protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-354 aa of BC065198 Sequence: MQMGDETRQRVKFTDDRVCKSHLLDCCPHDILAGTRMDLGECTKIHDLALRADYEIASKERDLFFELDAMDHLESFIAECDRRTELAKKRLAETQEEISAEVSAKAEKVHELNEEIGKLLAKAEQLGAEGNVDESQKILMEVEKVRAKKKEAEEEYRNSMPASSFQQQKLRVCEVCSAYLGLHDNDRRLADHFGGKLHLGFIQIREKLDQLRKTVAEKQEKRNQDRLRRREEREREERLSRRSGSRTRDRRRSRSRDRRRRRSRSTSRERRKLSRSRSRDRHRRHRSRSRSHSRGHRRASRDRSAKYKFSRERASREESWESGRSERGPPDWRLESSNGKMASRRSEEKEAGEI Predict reactive species Full Name: LUC7-like (S. cerevisiae) Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 38 kDa and 44 kDa GenBank Accession Number: BC065198 Gene Symbol: LUC7L Gene ID (NCBI): 55692 RRID: AB_3671199 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9NQ29 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.