Proteintech, 83606-5-RR, PI3 Kinase p85 Beta Recombinant monoclonal antibody

Size: 20ul / 100ul The PI3 Kinase p85 Beta (83606-5-RR) by Proteintech is a Recombinant antibody targeting PI3 Kinase p85 Beta in WB, IF/ICC, ELISA applications with reactivity to human, rat samples 83606-5-RR targets PI3 Kinase p85 Beta in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells Positive IF/ICC detected in: Jurkat cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information PIK3R2 is also named as PI3 Kinase p85 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317). Specification Tested Reactivity: human, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag14367 Product name: Recombinant human PIK3R2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-324 aa of BC014170 Sequence: YRHESLAQYNAKLDTRLLYPVSKYQQDQIVKEDSVEAVGAQLKVYHQQYQDKSREYDQLYEEYTRTSQELQMKRTAIEAFNETIKIFEEQGQTQEKCSKEYLERFRREGNEKEMQRILLNSERLKSRIAEIHESRTKLEQQLRAQASDNREIDKRMNSLKPDLMQLRKIRDQYLVWLTQKGARQKKINEWLGIKNETEDQYALMEDEDDLPHHEERTWYVGKINRTQAEEMLSGKRDGTFLIRESSQRGCYACSVVVDGDTKHCVIYRTATGFGFAEPYNLYGSLKELVLHYQHASLVQHNDALTVTLAHPVRAPGPGPPPAAR Predict reactive species Full Name: phosphoinositide-3-kinase, regulatory subunit 2 (beta) Calculated Molecular Weight: 728 aa, 82 kDa Observed Molecular Weight: 82 kDa GenBank Accession Number: BC014170 Gene Symbol: PI3 Kinase p85 Beta Gene ID (NCBI): 5296 RRID: AB_3671219 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: O00459 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.