Proteintech, 83655-3-RR, Leptin Recombinant monoclonal antibody

Size: 20ul / 100ul The Leptin (83655-3-RR) by Proteintech is a Recombinant antibody targeting Leptin in WB, IHC, ELISA applications with reactivity to rat samples 83655-3-RR targets Leptin in WB, IHC, ELISA applications and shows reactivity with rat samples. Tested Applications Positive WB detected in: rat adipose tissue Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. Leptin is the most critical hormone in the homeostatic regulation of energy balance among those so far discovered. Leptin primarily acts on the neurons of the mediobasal part of hypothalamus to regulate food intake, thermogenesis, and the blood glucose level. Leptin also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development. Specification Tested Reactivity: rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Fusion Protein Predict reactive species Full Name: leptin Calculated Molecular Weight: 19 kDa Observed Molecular Weight: 15-16 kDa GenBank Accession Number: NM_013076.3 Gene Symbol: Leptin Gene ID (NCBI): 25608 RRID: AB_3671263 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P50596 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.