Proteintech, 83660-1-RR, SOX4 Recombinant monoclonal antibody

Size: 20ul / 100ul The SOX4 (83660-1-RR) by Proteintech is a Recombinant antibody targeting SOX4 in WB, FC (Intra), ELISA applications with reactivity to human samples 83660-1-RR targets SOX4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, A431 cells, MCF-7 cells, SGC-7901 cells Positive FC (Intra) detected in: SH-SY5Y cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors that play key roles in the regulation of stemness, differentiation, progenitor cell development, and multiple developmental pathways. A single-exon gene encodes the SOX4 protein and contains a highly conserved high-mobility group (HMG) DNA binding domain related to the TCF/LEF family of transcription factors that play important roles in the Wnt pathway. SOX4 is expressed in stem cells, and modulates stem cell activation and likely also plays a role in stem cell maintenance, possibly through activation of expression of SOX2 (PMID: 23764001, 31445218). The calculated molecular weight of SOX4 is 47 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag26583 Product name: Recombinant human SOX4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 70-160 aa of BC072668 Sequence: SQIERRKIMEQSPDMHNAEISKRLGKRWKLLKDSDKIPFIREAERLRLKHMADYPDYKYRPRKKVKSGNANSSSSAAASSKPGEKGDKVGG Predict reactive species Full Name: SRY (sex determining region Y)-box 4 Calculated Molecular Weight: 474 aa, 47 kDa Observed Molecular Weight: 47 kDa GenBank Accession Number: BC072668 Gene Symbol: SOX4 Gene ID (NCBI): 6659 RRID: AB_3671267 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q06945 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.