Proteintech, 83683-6-RR, Beta-2-Microglobulin Recombinant monoclonal antibody

Size: 20ul / 100ul The Beta-2-Microglobulin (83683-6-RR) by Proteintech is a Recombinant antibody targeting Beta-2-Microglobulin in WB, IHC, IP, ELISA applications with reactivity to human samples 83683-6-RR targets Beta-2-Microglobulin in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, HepG2 cells, Raji cells, U-937 cells, Jurkat cells Positive IP detected in: A431 cells Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:400-1:1600 Background Information Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag4433 Product name: Recombinant human B2M protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 22-119 aa of BC032589 Sequence: QRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM Predict reactive species Full Name: beta-2-microglobulin Calculated Molecular Weight: 119 aa, 14 kDa Observed Molecular Weight: 12 kDa GenBank Accession Number: BC032589 Gene Symbol: B2M Gene ID (NCBI): 567 ENSEMBL Gene ID: ENSG00000166710 RRID: AB_3671284 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P61769 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.