Proteintech, 83688-4-RR, CD126/IL-6R alpha Recombinant monoclonal antibody

Size: 20ul / 100ul The CD126/IL-6R alpha (83688-4-RR) by Proteintech is a Recombinant antibody targeting CD126/IL-6R alpha in WB, ELISA applications with reactivity to mouse samples 83688-4-RR targets CD126/IL-6R alpha in WB, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R, the complex of IL-6 and IL-6R associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while IL-6R is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Fusion Protein Predict reactive species Full Name: interleukin 6 receptor Calculated Molecular Weight: 468 aa, 52 kDa Observed Molecular Weight: 65 kDA GenBank Accession Number: BC132684 Gene Symbol: IL-6R alpha Gene ID (NCBI): 3570 RRID: AB_3671290 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P08887 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.