Proteintech, 83690-5-RR, MARS Recombinant monoclonal antibody

Size: 20ul / 100ul The MARS (83690-5-RR) by Proteintech is a Recombinant antibody targeting MARS in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 83690-5-RR targets MARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, HepG2 cells, PC-12 cells, mouse testis tissue Positive IHC detected in: human ovarian cancer, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information The methionyl-tRNA synthetase (MARS) gene encodes cytoplasmic methionyl-tRNA synthetase (MetRS) responsible for catalyzing the ligation of methionine to tRNA. MetRS belongs to a family of aminoacyl-tRNA synthetases that play critical roles in protein biosynthesis by charging tRNAs with their cognate amino acids. MetRS overexpression was shown to be evident in human colon cancer patients. MetRS may thus be involved in oncogenic transformation. MetRS has been proven as a potential prognostic marker candidate for the clinical prognostic prediction of non-small-cell lung cancer (NSCLC) in patients. MetRS can also be used to detect intracellular oxidative stress and control protein synthesis under oxidative stress (PMID: 30271085, PMID: 34679529, PMID: 32404475). The calculated molecular weight of MetRS is 101 kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag6619 Product name: Recombinant human MARS protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 552-900 aa of BC002384 Sequence: MAKDNVPFHSLVFPCSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLLKNNSELLNNLGNFINRAGMFVSKFFGGYVPEMVLTPDDQRLLAHVTLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGSEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILLTNFLCTLPAGHQIGTVSPLFQKLENDQIESLRQRFGGGQAKTSPKPAVVETVTTAKPQQIQALMDEVTKQGNIVRELKAQKADKNEVAAEVAKLLDLKKQLAVAEGKPPEAPKGKKKK Predict reactive species Full Name: methionyl-tRNA synthetase Calculated Molecular Weight: 101 kDa Observed Molecular Weight: 101 kDa GenBank Accession Number: BC002384 Gene Symbol: MARS Gene ID (NCBI): 4141 RRID: AB_3671294 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P56192 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.