Proteintech, 83694-4-RR, MTMR9 Recombinant monoclonal antibody

Size: 20ul / 100ul The MTMR9 (83694-4-RR) by Proteintech is a Recombinant antibody targeting MTMR9 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 83694-4-RR targets MTMR9 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HCT 116 cells, HEK-293 cells, HeLa cells, HepG2 cells, U2OS cells Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells, A549 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information MTMR9 (Myotubularin-related protein 9) acts as an adapter for myotubularin-related phosphatases (PMID: 19038970; 22647598). MTMR9 can increase lipid phosphatase MTMR6 catalytic activity, specifically towards phosphatidylinositol 3,5-bisphosphate and MTMR6 binding affinity for phosphorylated phosphatidylinositols (PMID: 19038970; 22647598). It can also increase MTMR8 catalytic activity towards phosphatidylinositol 3-phosphate and stabilize MTMR8 protein levels (PMID: 22647598). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag34134 Product name: Recombinant human MTMR9 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 424-549 aa of BC022003 Sequence: MLFEHAYASQFGTFLGNNESERCKLKLQQKTMSLWSWVNQPSELSKFTNPLFEANNLVIWPSVAPQSLPLWEGIFLRWNRSSKYLDEAYEEMVNIIEYNKELQAKVNILRRQLAELETEDGMQESP Predict reactive species Full Name: myotubularin related protein 9 Calculated Molecular Weight: 63 kDa Observed Molecular Weight: ~60 kDa GenBank Accession Number: BC022003 Gene Symbol: MTMR9 Gene ID (NCBI): 66036 RRID: AB_3671297 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q96QG7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.