Proteintech, 83704-2-RR, TNPO3 Recombinant monoclonal antibody

Size: 20ul / 100ul The TNPO3 (83704-2-RR) by Proteintech is a Recombinant antibody targeting TNPO3 in WB, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 83704-2-RR targets TNPO3 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, C2C12 cells, NIH/3T3 cells, mouse brain tissue, rat thymus tissue Positive FC (Intra) detected in: HeLa cells, U2OS cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Transportin 3 (TNPO3) also known as IPO12, TRNSR, is a karyopherin β that works as a nuclear carrier shuttling, from the cytoplasm to the nucleus, the serine/arginine-rich proteins (PMID: 33452620). TNPO3 specifically mediates the nuclear import of splicing factor serine/arginine (SR) proteins, such as RBM4, SFRS1, and SFRS2, by recognizing phosphorylated SR domains (PMID: 11517331, 12628928). Mutations in TNPO3 are associated with Autosomal Dominant Limb-Girdle Muscular Dystrophy 2 (PMID: 23543484). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag8769 Product name: Recombinant human TNPO3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 508-857 aa of BC009923 Sequence: LDKITECLSELCSVQVMALKKLLSQEPSNGISSDPTVFLDRLAVIFRHTNPIVENGQTHPCQKVIQEIWPVLSETLNKHRADNRIVERCCRCLRFAVRCVGKGSAALLQPLVTQMVNVYHVHQHSCFLYLGSILVDEYGMEEGCRQGLLDMLQALCIPTFQLLEQQNGLQNHPDTVDDLFRLATRFIQRSPVTLLRSQVVIPILQWAIASTTLDHRDANCSVMRFLRDLIHTGVANDHEEDFELRKELIGQVMNQLGQQLVSQLLHTCCFCLPPYTLPDVAEVLWEIMQVDRPTFCRWLENSLKGLPKETTVGAVTVTHKQLTDFHKQVTSAEECKQVCWALRDFTRLFR Predict reactive species Full Name: transportin 3 Calculated Molecular Weight: 975 aa, 110 kDa Observed Molecular Weight: 104 kDa GenBank Accession Number: BC009923 Gene Symbol: TNPO3 Gene ID (NCBI): 23534 RRID: AB_3671306 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9Y5L0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.