Proteintech, 83718-5-RR, MIB1 Recombinant monoclonal antibody

Size: 20ul / 100ul The MIB1 (83718-5-RR) by Proteintech is a Recombinant antibody targeting MIB1 in WB, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 83718-5-RR targets MIB1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, DU 145 cells, HEK-293 cells, K-562 cells, C6 cells, mouse brain tissue, rat brain tissue Positive FC (Intra) detected in: HeLa cells, SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information MIB1(Mind bomb homolog 1) is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. MIB1, identified as an interactor of death associated protein kinase (DAPK), probably regulate apoptosis through ubiquitination and degradation of DAPK, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. MIB1 promotes proteasomal degradation of NRF2 and sensitizes lung cancer cells to ferroptosis(PMID: 34670864). In addition, MIB1 upregulates IQGAP1 and promotes pancreatic cancer progression by inducing ST7 degradation(PMID: 33793053). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag2507 Product name: Recombinant human MIB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 707-1006 aa of BC022403 Sequence: LRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPNLCKALAKCHKEKVSGQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCANLMKKCVQCRAVVERRVPFIMCCGGKSSEDATDDISSGNIPVLQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY Predict reactive species Full Name: mindbomb homolog 1 (Drosophila) Calculated Molecular Weight: 110 kDa Observed Molecular Weight: 110 kDa GenBank Accession Number: BC022403 Gene Symbol: MIB1 Gene ID (NCBI): 57534 RRID: AB_3671320 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q86YT6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.