Proteintech, 83742-5-RR, RAGE Recombinant monoclonal antibody

Size: 20ul / 100ul The RAGE (83742-5-RR) by Proteintech is a Recombinant antibody targeting RAGE in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 83742-5-RR targets RAGE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: rat lung tissue, mouse lung tissue Positive IHC detected in: mouse lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse lung tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information The receptor for advanced glycation end products (RAGE) is a cell surface transmembrane multiligand receptor, also known as Ager, is a multiligand member of the immunoglobulin superfamily. It has many isoforms and is predominantly expressed in the lung. AGER expression is upregulated in many diseases such as Alzheimer's disease, diabetes mellitus, atherosclerosis, and rheumatoid arthritis (PMID: 35099614). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: advanced glycosylation end product-specific receptor Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 42-55 kDa GenBank Accession Number: NM_007425.3 Gene Symbol: Ager Gene ID (NCBI): 11596 RRID: AB_3671340 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q62151-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.