Proteintech, 83751-6-RR, GBP1 Recombinant monoclonal antibody

Size: 20ul / 100ul The GBP1 (83751-6-RR) by Proteintech is a Recombinant antibody targeting GBP1 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 83751-6-RR targets GBP1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A2780 cells, HUVEC cells Positive IF/ICC detected in: IFN gamma HeLa cells Positive FC (Intra) detected in: IFN gamma (100ng/ml 24h) treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by interferon and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP. GBP1 is induced in response to type I and type II interferons and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus, and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag7562 Product name: Recombinant human GBP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 242-592 aa of BC002666 Sequence: VHRRKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIQVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVQKAIAHYEQQMGQKVQLPTESLQELLDLHRDSEREAIEVFIRSSFKDVDHLFQKELAAQLEKKRDDFCKQNQEASSDRCSGLLQVIFSPLEEEVKAGIYSKPGGYRLFVQKLQDLKKKYYEEPRKGIQAEEILQTYLKSKESMTDAILQTDQTLTEKEKEIEVERVKAESAQASAKMLQEMQRKNEQMMEQKERSYQEHLKQLTEKMENDRVQLLKEQERTLALKLQEQEQLLKEGFQKESRIMKNEIQDLQTKMRRRKACTIS Predict reactive species Full Name: GTP binding protein 1 Calculated Molecular Weight: 68 kDa Observed Molecular Weight: 67 kDa GenBank Accession Number: BC002666 Gene Symbol: GBP1 Gene ID (NCBI): 2633 RRID: AB_3671347 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P32455 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.