Proteintech, 83786-4-RR, SCPEP1 Recombinant monoclonal antibody

Size: 20ul / 100ul The SCPEP1 (83786-4-RR) by Proteintech is a Recombinant antibody targeting SCPEP1 in WB, IHC, ELISA applications with reactivity to human samples 83786-4-RR targets SCPEP1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: U-251 cells, A431 cells Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunohistochemistry (IHC): IHC : 1:300-1:1200 Background Information SCPEP1 encodes retinoid-inducible serine carboxypeptidase (PMID:12975309). SCPEP1 and Cathepsin A play roles in regulation of vasoconstriction via inactivation of endothelin-1 (PubMed:24586188). The protein is widely expressed during mouse embryogenesis by immunohistochemical (PubMed:16461364). Scpep1 is proteolytically cleaved to a mature enzyme which is smaller than 51 kDa (PMID:16461364). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag31381 Product name: Recombinant human SCPEP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 221-452 aa of BC072405 Sequence: LLEDKGLAEVSKVAEQVLNAVNKGLYREATELWGKAEMIIEQNTDGVNFYNILTKSTPTSTMESSLEFTQSHLVCLCQRHVRHLQRDALSQLMNGPIRKKLKIIPEDQSWGGQATNVFVNMEEDFMKPVISIVDELLEAGINVTVYNGQLDLIVDTMGQEAWVRKLKWPELPKFSQLKWKALYSDPKSLETSAFVKSYKNLAFYWILKAGHMVPSDQGDMALKMMRLVTQQE Predict reactive species Full Name: serine carboxypeptidase 1 Calculated Molecular Weight: 452 aa, 51 kDa Observed Molecular Weight: 33 kDa, 45~51 kDa GenBank Accession Number: BC072405 Gene Symbol: SCPEP1 Gene ID (NCBI): 59342 RRID: AB_3671378 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9HB40 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.