Proteintech, 83853-2-RR, Lipocalin-2/NGAL Recombinant monoclonal antibody

Size: 20ul / 100ul The Lipocalin-2/NGAL (83853-2-RR) by Proteintech is a Recombinant antibody targeting Lipocalin-2/NGAL in WB, IHC, ELISA applications with reactivity to mouse, rat samples 83853-2-RR targets Lipocalin-2/NGAL in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: 3T3-L1 cells, mouse testis tissue, rat lung tissue Positive IHC detected in: mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it was subsequently shown that many other types of cells, including those in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes rat Lipocalin-2/Ngal. Specification Tested Reactivity: mouse, rat Cited Reactivity: rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: lipocalin 2 Calculated Molecular Weight: 22kDa Observed Molecular Weight: 22-25 kDa Gene Symbol: Lipocalin-2/NGAL Gene ID (NCBI): 170496 RRID: AB_3671438 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P30152 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.