Proteintech, 83866-2-RR, Thyroid Hormone Receptor beta Recombinant monoclonal antibody

Size: 20ul / 100ul The Thyroid Hormone Receptor beta (83866-2-RR) by Proteintech is a Recombinant antibody targeting Thyroid Hormone Receptor beta in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 83866-2-RR targets Thyroid Hormone Receptor beta in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse pancreas tissue Positive IF/ICC detected in: A431 cells Positive FC (Intra) detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Thyroid hormone receptor beta (THRB), also known as ERBA2, NR1A2, and THR1, belongs to the nuclear hormone receptor family and NR1 subfamily. THRB is a nuclear hormone receptor that can act as a repressor or activator of transcription. Moreover, THRB is also a high-affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. THRB is the most abundant THR isoform in the liver but is also expressed in other tissues and mediates the effects of THs on hepatic carbohydrate and lipid metabolism (PMID: 34884910). THRB has 2 isoforms with the molecular mass of 53 and 54 kDa (refer to UniProt). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag16276 Product name: Recombinant human THRB protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 112-461 aa of BC106929 Sequence: DKATGYHYRCITCEGCKGFFRRTIQKNLHPSYSCKYEGKCVIDKVTRNQCQECRFKKCIYVGMATDLVLDDSKRLAKRKLIEENREKRRREELQKSIGHKPEPTDEEWELIKTVTEAHVATNAQGSHWKQKRKFLPEDIGQAPIVNAPEGGKVDLEAFSHFTKIITPAITRVVDFAKKLPMFCELPCEDQIILLKGCCMEIMSLRAAVRYDPESETLTLNGEMAVTRGQLKNGGLGVVSDAIFDLGMSLSSFNLDDTEVALLQAVLLMSSDRPGLACVERIEKYQDSFLLAFEHYINYRKHHVTHFWPKLLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFED Predict reactive species Full Name: thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian) Calculated Molecular Weight: 476 aa, 54 kDa Observed Molecular Weight: 53 kDa and 54 kDa GenBank Accession Number: BC106929 Gene Symbol: Thyroid Hormone Receptor beta Gene ID (NCBI): 7068 RRID: AB_3671452 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P10828 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.