Proteintech, 83872-1-RR, PRDM16 Recombinant monoclonal antibody

Size: 20ul / 100ul The PRDM16 (83872-1-RR) by Proteintech is a Recombinant antibody targeting PRDM16 in WB, IF/ICC, ELISA applications with reactivity to human samples 83872-1-RR targets PRDM16 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: U2OS cells, K-562 cells Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information PR domain containing 16, also known as PRDM16, acts as a transcription coregulator that controls the development of brown adipocytes in brown adipose tissue. Previously, this coregulator was believed to be present only in brown adipose tissue, but more recent studies have shown that PRDM16 is highly expressed in subcutaneous white adipose tissue as well. The protein encoded by this gene is a zinc finger transcription factor. PRDM16 controls the cell fate between muscle and brown fat cells. Loss of PRDM16 from brown fat precursors causes a loss of brown fat characteristics and promotes muscle differentiation. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: PR domain containing 16 Calculated Molecular Weight: 140 kDa Observed Molecular Weight: ~140 kDa GenBank Accession Number: NM_022114 Gene Symbol: PRDM16 Gene ID (NCBI): 63976 RRID: AB_3671458 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9HAZ2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.