Proteintech, 83946-4-RR, FANCD2 Recombinant monoclonal antibody

Size: 20ul / 100ul The FANCD2 (83946-4-RR) by Proteintech is a Recombinant antibody targeting FANCD2 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 83946-4-RR targets FANCD2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, K-562 cells, MCF-7 cells, Jurkat cells, U2OS cells Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Amongst the FA genes, Fanconi Anemia Group D2 Protein (FANCD2) is the most evolutionarily conserved gene, demonstrating a high sequence similarity from lower eukaryotes to humans. Upon activation, Fanconi anemia group D2 protein (FANCD2) is monoubiquitinated at K561 and works in cooperation with other FA and non-FA proteins to repair DNA damage. In regards to the FA pathway, monoubiquitinated or activated FANCD2 is often portrayed as the functional representative for the functions of activated FA signaling. (PMID: 28825622) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag29460 Product name: Recombinant human FANCD2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-127 aa of NM_001018115 Sequence: MVSKRRLSKSEDKESLTEDASKTRKQPLSKKTKKSHIANEVEENDSIFVKLLKISGIILKTGESQNQLAVDQIAFQKKLFQTLRRHPSYPKIIEEFVSGLESYIEDEDSFRNCLLSCERLQDEEASM Predict reactive species Full Name: Fanconi anemia, complementation group D2 Calculated Molecular Weight: 164 kDa Observed Molecular Weight: 150-160 kDa GenBank Accession Number: NM_001018115 Gene Symbol: FANCD2 Gene ID (NCBI): 2177 RRID: AB_3671526 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9BXW9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.