Proteintech, 84003-5-RR, HORMAD1 Recombinant monoclonal antibody

Size: 20ul / 100ul The HORMAD1 (84003-5-RR) by Proteintech is a Recombinant antibody targeting HORMAD1 in WB, FC (Intra), ELISA applications with reactivity to human samples 84003-5-RR targets HORMAD1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human testis tissue Positive FC (Intra) detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734]. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag4914 Product name: Recombinant human HORMAD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-394 aa of BC047406 Sequence: MATAQLQRTPMSALVFPNKISTEHQSLVLVKRLLAVSVSCITYLRGIFPECAYGTRYLDDLCVKILREDKNCPGSTQLVKWMLGCYDALQKKYLRMVVLAVYTNPEDPQTISECYQFKFKYTNNGPLMDFISKNQSNESSMLSTDTKKASILLIRKIYILMQNLGPLPNDVCLTMKLFYYDEVTPPDYQPPGFKDGDCEGVIFEGEPMYLNVGEVSTPFHIFKVKVTTERERMENIDSTILSPKQIKTPFQKILRDKDVEDEQEHYTSDDLDIETKMEEQEKNPASSELEEPSLVCEEDEIMRSKESPDLSISHSQVEQLVNKTSELDMSESKTRSGKVFQNKMANGNQPVKSSKENRKRSQHESGRIVLHHFDSSSQESVPKRRKFSEPKEHI Predict reactive species Full Name: HORMA domain containing 1 Calculated Molecular Weight: 45 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC047406 Gene Symbol: HORMAD1 Gene ID (NCBI): 84072 RRID: AB_3671574 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q86X24 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.