Proteintech, 84046-1-RR, HER2/ErbB2 Recombinant monoclonal antibody

Size: 20ul / 100ul The HER2/ErbB2 (84046-1-RR) by Proteintech is a Recombinant antibody targeting HER2/ErbB2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 84046-1-RR targets HER2/ErbB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells, NCI-H1299 cells, A549 cells, 4T1 cells, SGC-7901 cells Positive IHC detected in: human ovarian cancer, mouse uterus tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) Calculated Molecular Weight: 138 kDa Observed Molecular Weight: 185 kDa GenBank Accession Number: BC167147 Gene Symbol: HER2/ErbB2 Gene ID (NCBI): 2064 RRID: AB_3671613 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P04626 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.