Proteintech, 84063-1-RR, IDO1 Recombinant monoclonal antibody

Size: 20ul / 100ul The IDO1 (84063-1-RR) by Proteintech is a Recombinant antibody targeting IDO1 in WB, FC (Intra), ELISA applications with reactivity to human samples 84063-1-RR targets IDO1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: IFN gamma treated HeLa cells Positive FC (Intra) detected in: IFN-gamma treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag3953 Product name: Recombinant human IDO1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-403 aa of BC027882 Sequence: MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG Predict reactive species Full Name: indoleamine 2,3-dioxygenase 1 Calculated Molecular Weight: 403 aa, 45 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC027882 Gene Symbol: IDO1 Gene ID (NCBI): 3620 RRID: AB_3671632 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P14902 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.