Proteintech, 84086-1-RR, CD244 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD244 (84086-1-RR) by Proteintech is a Recombinant antibody targeting CD244 in WB, IHC, ELISA applications with reactivity to human samples 84086-1-RR targets CD244 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: NK-92 cells Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Fusion Protein Predict reactive species Full Name: CD244 molecule, natural killer cell receptor 2B4 Calculated Molecular Weight: 365 aa, 41 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC053985 Gene Symbol: CD244 Gene ID (NCBI): 51744 RRID: AB_3671654 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9BZW8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.