Proteintech, 84091-4-RR, TRPV4 Recombinant monoclonal antibody

Size: 20ul / 100ul The TRPV4 (84091-4-RR) by Proteintech is a Recombinant antibody targeting TRPV4 in WB, ELISA applications with reactivity to human, rat samples 84091-4-RR targets TRPV4 in WB, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: unboiled rat brain tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information TRPV4, also called VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found. Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag27253 Product name: Recombinant human TRPV4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-210 aa of NM_001177428.1 Sequence: MADSSEGPRAGPGEVAELPGDESGTPGGEAFPLSSLANLFEGEDGSLSPSPADASRPAGPGDGRPNLRMKFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPMDSLFDYGTYRHHSSDNKRWRKKIIEKQPQSPKAPAPQPPPILKVFNRPILFDIVSRGSTADLDGLLPFLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRNDTI Predict reactive species Full Name: transient receptor potential cation channel, subfamily V, member 4 Calculated Molecular Weight: 98 kDa Observed Molecular Weight: 85~98 kDa GenBank Accession Number: NM_001177428.1 Gene Symbol: TRPV4 Gene ID (NCBI): 59341 RRID: AB_3671657 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9HBA0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.