Proteintech, 84141-1-RR, GLI2-Specific Recombinant monoclonal antibody

Size: 20ul / 100ul The GLI2-Specific (84141-1-RR) by Proteintech is a Recombinant antibody targeting GLI2-Specific in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 84141-1-RR targets GLI2-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, SKOV-3 cells, HT-1080 cells, A549 cells, NIH/3T3 cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: GLI family zinc finger 2 Calculated Molecular Weight: 168 kDa Observed Molecular Weight: 80-90 kDa, 130-140 kDa, 160-180 kDa GenBank Accession Number: NM_005270 Gene Symbol: GLI2 Gene ID (NCBI): 2736 RRID: AB_3671702 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q0VGT2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.