Proteintech, 84147-1-RR, Phospho-JUN (Ser63) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-JUN (Ser63) (84147-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-JUN (Ser63) in WB, ELISA, ChIP-qPCR applications with reactivity to human, mouse samples 84147-1-RR targets Phospho-JUN (Ser63) in WB, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Anisomycin treated NIH/3T3 cells, UV treated NIH/3T3 cells Positive ChIP-qPCR detected in: hβ-NGF (50 ng/ml, 2 hr) PC-12 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis.The phosphorylation of c-Jun by Plk3 immunoprecipitates was not altered by the pre-removal of JNK from the cell lysates. In addition, the effect of UV irradiation-induced phosphorylation of c-Jun and apoptosis were not significantly affected by knockdown of JNK mRNA. (PMID: 22180088, PMID: 17804415) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: jun oncogene Calculated Molecular Weight: 331 aa, 36 kDa Observed Molecular Weight: 40-48 kDa GenBank Accession Number: BC068522 Gene Symbol: JUN Gene ID (NCBI): 3725 RRID: AB_3671710 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P05412 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.