Proteintech, 84233-8-RR, Carbonic Anhydrase IX/CA9 Recombinant monoclonal antibody

Size: 20ul / 100ul The Carbonic Anhydrase IX/CA9 (84233-8-RR) by Proteintech is a Recombinant antibody targeting Carbonic Anhydrase IX/CA9 in IHC, ELISA applications with reactivity to human samples 84233-8-RR targets Carbonic Anhydrase IX/CA9 in IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: human kidney(renal cell) cancer, human renal cell carcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia (PMID:18703501). It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma. The protein is present in the cytoplasmic membrane and the nucleus, with a molecular weight of 50 kDa. The molecular weight of the glycosylated form is approximately 60-70 kDa. (PMID: 31142270, PMID: 31819036) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag36941 Product name: Recombinant human CA9 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 32-408 aa of BC014950 Sequence: LMPVHPQRLPRMQEDSPLGGGSSGEDDPLGEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKPKSEEEGSLKLEDLPTVEAPGDPQEPQNNAHRDKEGDDQSHWRYGGDPPWPRVSPACAGRFQSPVDIRPQLAAFCPALRPLELLGFQLPPLPELRLRNNGHSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIAEEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCAQGVIWTVFNQTVMLSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRAAEPVQLNS Predict reactive species Full Name: carbonic anhydrase IX Calculated Molecular Weight: 459 aa, 50 kDa GenBank Accession Number: BC014950 Gene Symbol: CA9 Gene ID (NCBI): 768 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q16790 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.