Proteintech, 84261-5-RR, PKC Delta Recombinant monoclonal antibody

Size: 20ul / 100ul The PKC Delta (84261-5-RR) by Proteintech is a Recombinant antibody targeting PKC Delta in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 84261-5-RR targets PKC Delta in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: NIH/3T3 cells, mouse brain tissue, rat brain tissue Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HT-1080 cells, Hela cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information PKC Delta is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrom. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag5473 Product name: Recombinant human PRKCD protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 326-676 aa of BC043350 Sequence: DMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED Predict reactive species Full Name: protein kinase C, delta Calculated Molecular Weight: 78 kDa Observed Molecular Weight: 70-80 kDa GenBank Accession Number: BC043350 Gene Symbol: PKC Delta Gene ID (NCBI): 5580 RRID: AB_3671808 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q05655 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.