Proteintech, 84265-2-RR, CD22 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD22 (84265-2-RR) by Proteintech is a Recombinant antibody targeting CD22 in WB, ELISA applications with reactivity to human samples 84265-2-RR targets CD22 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Daudi cells, Raji cells, Ramos cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant Protein Predict reactive species Full Name: CD22 molecule Calculated Molecular Weight: 847 aa, 95 kDa Observed Molecular Weight: 130-140 kDa GenBank Accession Number: BC109306 Gene Symbol: CD22 Gene ID (NCBI): 933 RRID: AB_3671811 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P20273 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.