Proteintech, 84277-1-RR, CPS1 Recombinant monoclonal antibody

Size: 20ul / 100ul The CPS1 (84277-1-RR) by Proteintech is a Recombinant antibody targeting CPS1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 84277-1-RR targets CPS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, mouse liver tissue, rat liver tissue Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Carbamoyl phosphate synthetase 1 (CPS1) is the first rate-limiting enzyme in the urea cycle, converting ammonium into carbamoyl phosphate, and plays an intricate role in arginine metabolism and pyrimidine metabolism (PMID: 28376202). CPS1 expression is liver specific and suppressed in HCC cells and tumor tissues at the transcriptional level(PMID: 21281797). CPS1 gene comprises 38 exons that encode a polypeptide of 1500 amino acids and has a molecular weight of 165 kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: carbamoyl-phosphate synthetase 1, mitochondrial Calculated Molecular Weight: 165 kDa Observed Molecular Weight: 140-165 kDa GenBank Accession Number: BC140943 Gene Symbol: CPS1 Gene ID (NCBI): 1373 RRID: AB_3671823 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P31327 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.