Proteintech, 84310-2-RR, CCT5 Recombinant monoclonal antibody

Size: 20ul / 100ul The CCT5 (84310-2-RR) by Proteintech is a Recombinant antibody targeting CCT5 in WB, IF/ICC, ELISA applications with reactivity to human samples 84310-2-RR targets CCT5 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, HEK-293T cells, HeLa cells, Jurkat cells, A549 cells, COLO 320 cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC).CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag2146 Product name: Recombinant human CCT5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 243-541 aa of BC035499 Sequence: VEDAKIAILTCPFEPPKPKTKHKLDVTSVEDYKALQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIAIATGGRIVPRFSELTAEKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNKMIIEEAKRSLHDALCVIRNLIRDNRVVYGGGAAEISCALAVSQEADKCPTLEQYAMRAFADALEVIPMALSENSGMNPIQTMTEVRARQVKEMNPALGIDCLHKGTNDMKQQHVIETLIGKKQQISLATQMVRMILKIDDIRKPGESEE Predict reactive species Full Name: chaperonin containing TCP1, subunit 5 (epsilon) Calculated Molecular Weight: 541 aa, 60 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC035499 Gene Symbol: CCT5 Gene ID (NCBI): 22948 RRID: AB_3671850 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P48643 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.