Proteintech, 84327-1-RR, CLEC14A Recombinant monoclonal antibody

Size: 20ul / 100ul The CLEC14A (84327-1-RR) by Proteintech is a Recombinant antibody targeting CLEC14A in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 84327-1-RR targets CLEC14A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293T cells, HUVEC cells, mouse brain tissue, rat brain tissue Positive IHC detected in: human placenta tissue, human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information CLEC14A is a single-pass type I transmembrane protein whose extracellular domain consists of a C-type lectin-like domain (CTLD), a series of EGF-like domains, and a sushi-like domain (PMID: 23644659). CLEC14A is a member of the C-type lectin domain superfamily that also includes endosialin, thrombomodulin and CD93 (PMID: 16336259). It has been reported that CLEC14A is specifically expressed in endothelial cells and mediates cell to cell adhesion (PMID: 21095181). CLEC14A may be involved in tumor angiogenesis (PMID: 21706054; 23644659). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag21081 Product name: Recombinant human CLEC14A protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-349 aa of BC031567 Sequence: MRPAFALCLLWQALWPGPGGGEHPTADRAGCSASGACYSLHHATMKRQAAEEACILRGGALSTVRAGAELRAVLALLRAGPGPGGGSKDLLFWVALERRRSHCTLENEPLRGFSWLSSDPGGLESDTLQWVEEPQRSCTARRCAVLQATGGVEPAGWKEMRCHLRANGYLCKYQFEVLCPAPRPGAASNLSYRAPFQLHSAALDFSPPGTEVSALCRGQLPISVTCIADEIGARWDKLSGDVLCPCPGRYLRAGKCAELPNCLDDLGGFACECATGFELGKDGRSCVTSGEGQPTLGGTGVPTRRPPATATSPVPQRTWPIRVDEKLGETPLVPEQDNSVTSIPEIPRW Predict reactive species Full Name: C-type lectin domain family 14, member A Calculated Molecular Weight: 490 aa, 52 kDa Observed Molecular Weight: 52 kDa, 100 kDa GenBank Accession Number: BC031567 Gene Symbol: CLEC14A Gene ID (NCBI): 161198 RRID: AB_3671868 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q86T13 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.